Does it matter which slot you put an additional RAM card in?

Which two RAM slots to populate on a 4-slot system? ram slot 2 and 4 Test 4: Slot 1: Empty Slot 2: Samsung RAM Result: Power button light boots but screen won't turn on Test 5: Slot 1: Crucial RAM SLot 2: Empty rampage poker player It depends on how much RAM you want and how many DIMMs you'll use to get there 16GB of RAM should do the trick if you're mostly focused on

ramses legacy slot 2 USB ports; 2 USB ports Raspberry Pi standard 40 pin GPIO header Micro-SD card slot for loading operating system and data storage; 5V DC